Difference between revisions of "Part:BBa K2729008"

 
 
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<partinfo>BBa_K2729008 short</partinfo>
 
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YFP is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
 
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===Usage and Biology===
 
===Usage and Biology===
  
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ZsYellow is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729008 SequenceAndFeatures</partinfo>
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===Characterization===
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The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)'''  ]]
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As shown in Figure 1, the band comes between 900 bp to 1,000 bp. The actual length without prefix and suffix is 920 bp.
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===Analysis===
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
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The function of FMDV2a will be tested by testing the infection of pseudo-virus into Vero cells. If the 2a peptide works well, the fluorescence intensity can be observed robustly.
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===Sequence and Features===
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<partinfo>BBa_K2729008 SequenceAndFeatures</partinfo>
  
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===Reference===
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===Functional Parameters===
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- [https://web.stanford.edu/class/chem184/lectures08/Kool_Photochem.pdf Stanford University Chemistry of ZS Yellow fluorophore]
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<partinfo>BBa_K2729008 parameters</partinfo>
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Latest revision as of 03:22, 18 October 2018


ZsYellow-FMDV 2A

Usage and Biology

ZsYellow is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance.

Characterization

The sequence is optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band comes between 900 bp to 1,000 bp. The actual length without prefix and suffix is 920 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

The function of FMDV2a will be tested by testing the infection of pseudo-virus into Vero cells. If the 2a peptide works well, the fluorescence intensity can be observed robustly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 820
    Illegal AgeI site found at 632
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

- Stanford University Chemistry of ZS Yellow fluorophore