Difference between revisions of "Part:BBa K2729007"
 
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The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
 
The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
  
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[[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]]
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[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
  
 
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As shown in Figure 1, the band comes around 900 bp.  
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As shown in Figure 1, the band comes around 900 bp. The actual length without prefix and suffix is 908 bp.
 
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
 
Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
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The function of FMDV2a will be tested by testing the infection of pseudo-virus into Vero cells. If the 2a peptide works well, the fluorescence intensity can be observed robustly.
  
 
===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K2729007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2729007 SequenceAndFeatures</partinfo>
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===Reference===
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-[https://www.takarabio.com/products/gene-function/fluorescent-proteins/fluorescent-protein-plasmids/red-fluorescent-proteins/dsred-express-and-dsred-express2-fluorescent-proteins Takara Bio - DsRed-Express]

Latest revision as of 03:17, 18 October 2018


DsRed-Express-FMDV 2A

Usage and Biology

DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance.

Characterization

The sequence is optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band comes around 900 bp. The actual length without prefix and suffix is 908 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

The function of FMDV2a will be tested by testing the infection of pseudo-virus into Vero cells. If the 2a peptide works well, the fluorescence intensity can be observed robustly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 808
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

-Takara Bio - DsRed-Express