Difference between revisions of "Part:BBa K2729007"
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DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. | DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. | ||
| − | FMDV 2A is | + | FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance. |
===Characterization=== | ===Characterization=== | ||
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The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it. | The sequence is optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it. | ||
| − | [[File: | + | [[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]] |
<br> | <br> | ||
| − | As shown in Figure 1, the band comes around 900 bp. | + | As shown in Figure 1, the band comes around 900 bp. The actual length without prefix and suffix is 908 bp. |
<br style="clear: both" /> | <br style="clear: both" /> | ||
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection. | Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection. | ||
| + | |||
| + | The function of FMDV2a will be tested by testing the infection of pseudo-virus into Vero cells. If the 2a peptide works well, the fluorescence intensity can be observed robustly. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K2729007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2729007 SequenceAndFeatures</partinfo> | ||
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| + | ===Reference=== | ||
| + | -[https://www.takarabio.com/products/gene-function/fluorescent-proteins/fluorescent-protein-plasmids/red-fluorescent-proteins/dsred-express-and-dsred-express2-fluorescent-proteins Takara Bio - DsRed-Express] | ||
Latest revision as of 03:17, 18 October 2018
DsRed-Express-FMDV 2A
Usage and Biology
DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance.
Characterization
The sequence is optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
As shown in Figure 1, the band comes around 900 bp. The actual length without prefix and suffix is 908 bp.
Analysis
Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.
The function of FMDV2a will be tested by testing the infection of pseudo-virus into Vero cells. If the 2a peptide works well, the fluorescence intensity can be observed robustly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 43
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 808
- 1000COMPATIBLE WITH RFC[1000]