Difference between revisions of "Part:BBa K2669003:Design"
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===Source=== | ===Source=== | ||
− | Biobrick was ordered as a customized | + | The sequence was codon optimized with the codon optimization tool provided by IDT. |
+ | |||
+ | Biobrick was ordered as a customized genes from Integrated DNA Technologies (IDT) | ||
constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014). | constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014). | ||
===References=== | ===References=== |
Latest revision as of 03:15, 18 October 2018
Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
The sequence was codon optimized with the codon optimization tool provided by IDT.
Biobrick was ordered as a customized genes from Integrated DNA Technologies (IDT)
constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).