Difference between revisions of "Part:BBa K2719003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence of GST from Schistosoma japonicum, was optimized for its expression on E.coli BL21 (DE3). This part is useful, as by fussing a protein on the N-terminus, it improves its stability, solubility and allows its purification by affinity column. | + | The sequence of GST from Schistosoma japonicum, was optimized for its expression on <i>E.coli</i> BL21 (DE3). This part is useful, as by fussing a protein on the N-terminus, it improves its stability, solubility and allows its purification by affinity column. |
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| + | "https://static.igem.org/mediawiki/2018/4/4f/T--TecCEM--GST3DStructure.png" | ||
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| + | <p><i>Figure 1</i>. GST 3D structure, modeled with Swiss-Model </p> | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Harper S. & Speicher D. (2010). Purification of proteins fused to glutathione S-transferase. Protein Chromatography . Doi: 10.1007/978-1-60761-913-0_14. | ||
Latest revision as of 03:05, 18 October 2018
GST (Optimized for E.coli BL21)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 346
Design Notes
The sequence of GST from Schistosoma japonicum, was optimized for its expression on E.coli BL21 (DE3). This part is useful, as by fussing a protein on the N-terminus, it improves its stability, solubility and allows its purification by affinity column.
"
"
Figure 1. GST 3D structure, modeled with Swiss-Model
Source
Synthetic part.
References
Harper S. & Speicher D. (2010). Purification of proteins fused to glutathione S-transferase. Protein Chromatography . Doi: 10.1007/978-1-60761-913-0_14.