Difference between revisions of "Part:BBa K2719001:Experience"
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===Applications of BBa_K2719001=== | ===Applications of BBa_K2719001=== | ||
| − | <p>To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1)</p> | + | <p>To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in <i>Escherichia coli</i> DH5a. (Figure 1)</p> |
| − | [[file:T--TecCEM--OPTCDColony.png]] | + | [[file:T--TecCEM--OPTCDColony.png|500px]] |
| − | <p><i>Figure 1.</ | + | <p><i>Figure 1.</i> Colonies transformed with Tenascin 5 Domain V</p> |
| − | <p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure | + | <p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid extraction (Figure 2) and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 3)</p> |
| + | [[file:T--TecCEM--OPTCDEx.png|500px]] | ||
| + | <p><i>Figure 2.</i> Tenascin 5 Domain 085% Agarose Gel with GelRed: MW) 1Kb plus from NEB; 6) Tenascin 5 Domain (BBa_K2719001) </p> | ||
| + | |||
| + | [[file:T--TecCEM--OPTCDRES.png|500px]] | ||
| + | <p><i>Figure 3.</i> Tenascin 5 Domain V 0.85% agarose gel with GelRed: MW) 1KB plus from NEB; 4) Tenascin 5 Domain V (BBa_K2719001) </p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 02:43, 18 October 2018
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Applications of BBa_K2719001
To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1)
Figure 1. Colonies transformed with Tenascin 5 Domain V
To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid extraction (Figure 2) and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 3)
Figure 2. Tenascin 5 Domain 085% Agarose Gel with GelRed: MW) 1Kb plus from NEB; 6) Tenascin 5 Domain (BBa_K2719001)
Figure 3. Tenascin 5 Domain V 0.85% agarose gel with GelRed: MW) 1KB plus from NEB; 4) Tenascin 5 Domain V (BBa_K2719001)
User Reviews
UNIQ47e527a98bac5bd0-partinfo-00000000-QINU UNIQ47e527a98bac5bd0-partinfo-00000001-QINU