Difference between revisions of "Part:BBa K2876011"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2876011 short</partinfo> | <partinfo>BBa_K2876011 short</partinfo> | ||
| − | + | This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused this antibody to a lambda repressor (BBa_K2876001) (https://parts.igem.org/Part:BBa_K2876001) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000). | |
| + | |||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:40, 18 October 2018
IL-1 Beta scFv2
This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused this antibody to a lambda repressor (BBa_K2876001) (https://parts.igem.org/Part:BBa_K2876001) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]