Difference between revisions of "Part:BBa K2588024"

 
 
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<p>This part consists of the genes <a href="https://parts.igem.org/Part:BBa_K2588015">LeuABCD</a>, <a href="https://parts.igem.org/Part:BBa_K2588016">kivD</a> and <a href="https://parts.igem.org/Part:BBa_K2588017">ADH2</a>. It produces 3-methyl-1-butanol according to the pathway seen in fig. 1.<sup>1</sup></p>
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<img src="https://static.igem.org/mediawiki/parts/6/62/3MB.png">
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<p><b>Fig 1:</b> Metabolic pathway for amino acid biosynthesis and 3-methyl-1-butanol production. All genes are from <i>E. coli</i> unless otherwise noted. BS <i>B. subtilis</i>, LL <i>L. lactis</i>, SC <i>S. cerevisiae</i>.<sup>1</sup></p>
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<h2>References</h2>
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<li>Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. <i>Appl. Microbiol. Biotechnol.</i> <b>86</b>, 1155–1164 (2010).</li>
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Latest revision as of 02:35, 18 October 2018


3-methyl-1-butanol synthesis module, glucose-induced

This part consists of the genes LeuABCD, kivD and ADH2. It produces 3-methyl-1-butanol according to the pathway seen in fig. 1.1

Fig 1: Metabolic pathway for amino acid biosynthesis and 3-methyl-1-butanol production. All genes are from E. coli unless otherwise noted. BS B. subtilis, LL L. lactis, SC S. cerevisiae.1

References

  1. Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86, 1155–1164 (2010).

      2. Sequence and Features


      Assembly Compatibility:
      • 10
        COMPATIBLE WITH RFC[10]
      • 12
        INCOMPATIBLE WITH RFC[12]
        Illegal NheI site found at 144
        Illegal NheI site found at 167
      • 21
        INCOMPATIBLE WITH RFC[21]
        Illegal BamHI site found at 7747
        Illegal XhoI site found at 570
      • 23
        COMPATIBLE WITH RFC[23]
      • 25
        INCOMPATIBLE WITH RFC[25]
        Illegal NgoMIV site found at 1328
        Illegal AgeI site found at 6485
      • 1000
        INCOMPATIBLE WITH RFC[1000]
        Illegal SapI site found at 7573
        Illegal SapI.rc site found at 4405