Difference between revisions of "Part:BBa K2787009"

(→‎Usage and Biology)
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[[File:ShanghaiTech2018-A2.png|700px|center]]
 
[[File:ShanghaiTech2018-A2.png|700px|center]]
 
[[File:A2-B8-C9-Orthogonality.png|500px|center]]
 
[[File:A2-B8-C9-Orthogonality.png|500px|center]]
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[[File:ShanghaiTech2018-A2 A2.png|450px|center<b>Fig. 2 A2 O-16S fluorescence over time</b>]][[File:Shanghaitech-A2.png|450px|center<b>Fig. 3 A2 O-16S fluorescence at 200 minutes</b>]]<br>
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[[File:Shanghaitech-A2.png|450px|center<b>Fig. 3 A2 O-16S fluorescence at 200 minutes</b>]]<br>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 02:34, 18 October 2018


GFP under A2 orthogonal RBS with orthogonal 16S rRNA A2

The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set A2.

Usage and Biology

a plasmid with GFP under control of A2 orthogonal RBS with A2 orthogonal 16s rRNA is constructed to test the orthogonality of B8 RBS.

700px
ShanghaiTech2018-A2 A2.png
ShanghaiTech2018-A2 rbs.png
700px
A2-B8-C9-Orthogonality.png

centerFig. 3 A2 O-16S fluorescence at 200 minutes
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706