Difference between revisions of "Part:BBa K2725013"
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<partinfo>BBa_K2725013 short</partinfo> | <partinfo>BBa_K2725013 short</partinfo> | ||
| − | P1003 Kanamycin resistance cassette with 10 serine codons in the coding sequence substituted for amber stop codons (TAG): S17Amb, S36Amb, S60Amb, S116Amb, S133Amb, S154Amb, S189Amb, S200Amb, S240Amb, S242Amb. When transformed into E .coli there is no P1003 expression and no kanamycin resistance showing that 10 amber substitutions effectively prevents expression. | + | P1003 Kanamycin resistance cassette with 10 serine codons in the coding sequence substituted for amber stop codons (TAG): S17Amb, S36Amb, S60Amb, S116Amb, S133Amb, S154Amb, S189Amb, S200Amb, S240Amb, S242Amb. When transformed into E .coli there is no P1003 expression and no kanamycin resistance showing that 10 amber substitutions effectively prevents expression. This part is an improvement upon BBa_K914018 (https://parts.igem.org/Part:BBa_K914018) |
https://static.igem.org/mediawiki/2018/e/ec/T--Edinburgh_UG--400.png | https://static.igem.org/mediawiki/2018/e/ec/T--Edinburgh_UG--400.png | ||
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| + | Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin. | ||
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| + | https://static.igem.org/mediawiki/2018/9/96/T--Edinburgh_UG--prob_per_stop.png | ||
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| + | Figure 2. Graph showing the probability of a read-through (and therefore expression) of a gene with number of amber STOP codons within the coding sequence of the gene. When there are 10 amber STOP codons in the coding sequence 1 in every 10^18 translation attempts may result in expression of the gene. | ||
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Latest revision as of 02:23, 18 October 2018
P1003 Casette with 10 Amber substitutions
P1003 Kanamycin resistance cassette with 10 serine codons in the coding sequence substituted for amber stop codons (TAG): S17Amb, S36Amb, S60Amb, S116Amb, S133Amb, S154Amb, S189Amb, S200Amb, S240Amb, S242Amb. When transformed into E .coli there is no P1003 expression and no kanamycin resistance showing that 10 amber substitutions effectively prevents expression. This part is an improvement upon BBa_K914018 (https://parts.igem.org/Part:BBa_K914018)
Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin.
Figure 2. Graph showing the probability of a read-through (and therefore expression) of a gene with number of amber STOP codons within the coding sequence of the gene. When there are 10 amber STOP codons in the coding sequence 1 in every 10^18 translation attempts may result in expression of the gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]