Difference between revisions of "User:Scmohr/Antibiotic-res cat page"
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| + | This page contains a list of the contents for an antibiotic-resistance "catalog" page. The actual layout will be developed on [[User:Scmohr/Antibiotic-res_cat_page2 | New Catalog Page for Antibiotic Resistance Parts]]. | ||
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==Introduction to Antibiotic Resistance== | ==Introduction to Antibiotic Resistance== | ||
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More details about antibiotic resistance can be found at __________________. | More details about antibiotic resistance can be found at __________________. | ||
| − | ==Antibiotic-Resistance Basic Parts by Category== | + | ==Antibiotic-Resistance Key Basic Parts by Category== |
''NB'' Parts listed in '''boldface''' are "good" parts ["Available" and (in most cases) "Work"]. Parts in plain type are poorly characterized. Letters in parentheses () refer to the specific antibiotic that relates to the part. See | ''NB'' Parts listed in '''boldface''' are "good" parts ["Available" and (in most cases) "Work"]. Parts in plain type are poorly characterized. Letters in parentheses () refer to the specific antibiotic that relates to the part. See | ||
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====5. Components of Alternative Metabolic Pathways==== | ====5. Components of Alternative Metabolic Pathways==== | ||
| − | ==Antibiotic | + | ====Parts Supplementary to Key Antibiotic Resistance Parts==== |
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| + | Tet repressor; Tet promoter.... | ||
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| + | ==Devices Using Antibiotic-Resistance Parts by Category== | ||
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====3. With Membrane-embedded Efflux Pumps==== | ====3. With Membrane-embedded Efflux Pumps==== | ||
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====4. With Altered Target Molecules==== | ====4. With Altered Target Molecules==== | ||
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WHAT ABOUT THE VARIOUS CELL LINES OFFERED AS CHASSIS? | WHAT ABOUT THE VARIOUS CELL LINES OFFERED AS CHASSIS? | ||
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==Miscellaneous Information about Antibiotic Resistance in the Registry== | ==Miscellaneous Information about Antibiotic Resistance in the Registry== | ||
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:BBa_V1016 | :BBa_V1016 | ||
:BBa_V1019 | :BBa_V1019 | ||
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| + | ===2. Items with Membrane-blocking Proteins=== | ||
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| + | ===3. Items with Membrane-embedded Efflux Pumps=== | ||
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| + | ====Plasmids==== | ||
| + | :[https://parts.igem.org/wiki/index.php?title=Part:BBa_J69602 BBa_J69602] | ||
| + | |||
| + | ===4. Items with Altered Target Molecules=== | ||
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| + | ===5. Containing Components of Alternative Metabolic Pathways=== | ||
Latest revision as of 16:11, 31 July 2008
This page contains a list of the contents for an antibiotic-resistance "catalog" page. The actual layout will be developed on New Catalog Page for Antibiotic Resistance Parts.
Contents
Introduction to Antibiotic Resistance
The established mechanisms of antibiotic resistance include the following:
- Enzymes that inactivate the antibiotic [famously (a). beta-lactamases that destroy penicillins and related compounds and (b) enzymes that acetylate chloramphenicol].
- Proteins that keep antibiotics out of the cell (by blocking the outer-membrane pores).
- Membrane-embedded channel proteins that actively pump antibiotics out of the cell.
- Proteins (and RNAs) with altered drug binding sites. (Vancomycin resistance comes from a switch in the chemistry of the cell-wall cross-linking peptide). This category also includes mutations in ribosomal RNA -- ribosomes are one of the main targets of both prokaryote- and eukaryote-directed antibiotics.
- Proteins that enable substitution of an alternative metabolic pathway (as in the case of sulfonamide resistance).
More details about antibiotic resistance can be found at __________________.
Antibiotic-Resistance Key Basic Parts by Category
NB Parts listed in boldface are "good" parts ["Available" and (in most cases) "Work"]. Parts in plain type are poorly characterized. Letters in parentheses () refer to the specific antibiotic that relates to the part. See Plasmid/Nomenclature for definitions of the abbreviations.
1. Antibiotic-inactivating Enzymes
- BBa_J23012(S)
- BBa_J31002(K)
- BBa_J31003(K)
- BBa_J31004(C)
- BBa_J31005(C)
- BBa_J42020(S/ST)
- BBa_J42021(S/ST)
2. Membrane-blocking Proteins
3. Membrane-embedded Efflux Pumps
- BBa_J31006(T)
- BBa_J31007(T)
- BBa_R0040(T)
4. Altered Target Molecules
- BBa_J42010(St)
5. Components of Alternative Metabolic Pathways
Parts Supplementary to Key Antibiotic Resistance Parts
Tet repressor; Tet promoter....
Devices Using Antibiotic-Resistance Parts by Category
These are BioBrick composite parts that contain (one or more?) antibiotic resistance components.
1. With Antibiotic-inactivating Enzymes
2. With Membrane-blocking Proteins
3. With Membrane-embedded Efflux Pumps
4. With Altered Target Molecules
5. Containing Components of Alternative Metabolic Pathways
Note: Antibiotic resistance genes are present in most of the plasmids supplied by the Registry (see Plasmid).
WHAT ABOUT THE VARIOUS CELL LINES OFFERED AS CHASSIS?
Miscellaneous Information about Antibiotic Resistance in the Registry
1. Items with Antibiotic-inactivating Enzymes
Plasmids
The 98 well-characterized, available plasmids in the Registry, have the following resistance characteristics:
- Ampicillin(A) -- 54
- Chloramphenicol(C) -- 9
- Gentamycin -- 0
- Kanamycin(K)-- 9
- Tetracycline(T)-- 2
- AK -- 18
- AC -- 2
- AT -- 2
- AG -- 1
- AGK -- 1
- BBa_K125000
Cells
- BBa_V1016
- BBa_V1019