Difference between revisions of "Part:BBa K2725012"

Revision as of 02:20, 18 October 2018

P1003 Cassette with 5 amber stop codons

A P1003 kanamycin resistance cassette with 5 serine -> amber stop codon (TAG) substitutions: S36Amb, S133Amb, S154Amb, S200Amb, S240Amb. This gives kanamycin resistance when co-transformed with BBa_K2725016. This part demonstrates functionality of the semantic containment system for preventing horizontal gene transfer. This part is an improvement upon BBa_K914009(https://parts.igem.org/Part:BBa_K914009).

Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin.

Figure 2. Graph showing the probability of a read-through (and therefore expression) of a gene with number of amber STOP codons within the coding sequence of the gene. When there are 5 amber STOP codons in the coding sequence 1 in every 10¹⁰ translation attempts may result in expression of the gene.

Sequence and Features