Difference between revisions of "Part:BBa K2588011"
 
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<h2>Usage and Biology</h2>
 
<h2>Usage and Biology</h2>
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<p>The Acetyl-CoA biotin carboxyl carrier protein is part of the acetyl-CoA carboxylase ACC, which is the major regulator for fatty acid biosynthesis in e. coli. It is encoded as a single mRNA with the downstream accC, which is encoding for the biotin carboxylase of the complex.<sup>1</sup> The acetyl-CoA biotin carboxyl carrier protein binds with biotin and transfers carboxylated biotin to the acetyl-CoA carboxyltransferase.<sup>2</sup>
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<p>The Acetyl-CoA biotin carboxyl carrier protein is part of the acetyl-CoA carboxylase ACC which is the major regulator for fatty acid biosynthesis in <i>E. coli</i>. It is encoded as a single mRNA with the downstream accC, which is encoding for the biotin carboxylase of the complex.<sup>1</sup> The acetyl-CoA biotin carboxyl carrier protein binds with biotin and transfers carboxylated biotin to the acetyl-CoA carboxyltransferase.<sup>2</sup>
 
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<p>The acetyl-CoA biotin carboxyl carrier protein was used in our myristic acid synthesis module, <a href="https://parts.igem.org/Part:BBa_K2588022">BBa_K2588022</a>.</p>
 
<p>The acetyl-CoA biotin carboxyl carrier protein was used in our myristic acid synthesis module, <a href="https://parts.igem.org/Part:BBa_K2588022">BBa_K2588022</a>.</p>

Latest revision as of 02:15, 18 October 2018


Acetyl-CoA biotin carboxyl carrier protein

Usage and Biology

The Acetyl-CoA biotin carboxyl carrier protein is part of the acetyl-CoA carboxylase ACC which is the major regulator for fatty acid biosynthesis in E. coli. It is encoded as a single mRNA with the downstream accC, which is encoding for the biotin carboxylase of the complex.1 The acetyl-CoA biotin carboxyl carrier protein binds with biotin and transfers carboxylated biotin to the acetyl-CoA carboxyltransferase.2

The acetyl-CoA biotin carboxyl carrier protein was used in our myristic acid synthesis module, BBa_K2588022.

References

  1. Li, S. J. & Cronan, J. E. The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267, 855–63 (1992).
  2. Broussard, T. C., Price, A. E., Laborde, S. M. & Waldrop, G. L. Complex formation and regulation of escherichia coli acetyl-CoA carboxylase. Biochemistry (2013). doi:10.1021/bi4000707


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 432
  • 1000
    COMPATIBLE WITH RFC[1000]