Difference between revisions of "Part:BBa K2787009"

(Usage and Biology)
(Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
[[File:Shanghaitech layout7.png|700px|center]]
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[[File:ShanghaiTech2018 A2 16s.png|700px|center]]
[[File:Shanghaitech layout5.png|700px|center]]
+
[[File:ShanghaiTech2018 A2 A2.png|700px|center]]
 +
[[File:ShanghaiTech2018 A2 rbs.png|700px|center]]
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[[File:ShanghaiTech2018 A2.png|700px|center]]
 
[[File:A2-B8-C9-Orthogonality.png|500px|center]]
 
[[File:A2-B8-C9-Orthogonality.png|500px|center]]
 
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Revision as of 02:09, 18 October 2018


GFP under A2 orthogonal RBS with orthogonal 16S rRNA A2

The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to justify the proper function of the ribosomal 16SRNA and RBS set A2.

Usage and Biology

A2-B8-C9-Orthogonality.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 797
    Illegal NheI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706