Difference between revisions of "Part:BBa K2333009"
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mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes. | mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes. | ||
| − | <!-- Add more about the biology of this part here | + | ===Improvement=== |
| + | An improved mScarlet-I is available in part <partinfo>BBa_K2680251</partinfo>. This part is compatible with 3G assembly and can be used with William and Mary 2018's <html><a href = 'http://2018.igem.org/Team:William_and_Mary/3G_Protocols'>protocols </a></html>, to produce large numbers of multi-transcriptional unit circuits and circuit variants. | ||
| + | |||
| + | <!-- Add more about the biology of this part here> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 02:03, 18 October 2018
mScarlet-I
mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes.
Improvement
An improved mScarlet-I is available in part BBa_K2680251. This part is compatible with 3G assembly and can be used with William and Mary 2018's protocols , to produce large numbers of multi-transcriptional unit circuits and circuit variants.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]