Difference between revisions of "Part:BBa K2550001:Experience"
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| − | <p>The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented darker blue colonies in comparison to the single switch transformation confirming the function of the trigger as it induces the toehold switch to produce blue pigment in response to the presence of Xgal in conjunction with the LacZ reporter gene. In addition, sequencing results shown below also confirms the presence | + | <p>The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented darker blue colonies in comparison to the single switch transformation confirming the function of the trigger as it induces the toehold switch to produce blue pigment in response to the presence of Xgal in conjunction with the LacZ reporter gene. In addition, sequencing analysis results shown below also confirms the presence of the trigger in comparison to the trigger sequences in the reference paper (Green et al. 2014). The biobricked trigger's (BBa_K2550001) function has been validated through experimentation and sequencing. </p> |
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<center><img src="https://static.igem.org/mediawiki/parts/d/d8/T--LambertGA--TRIGGER.png" height="350 px"></center> | <center><img src="https://static.igem.org/mediawiki/parts/d/d8/T--LambertGA--TRIGGER.png" height="350 px"></center> | ||
Revision as of 02:03, 18 October 2018
Applications of BBa_K2550001
Results
The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented darker blue colonies in comparison to the single switch transformation confirming the function of the trigger as it induces the toehold switch to produce blue pigment in response to the presence of Xgal in conjunction with the LacZ reporter gene. In addition, sequencing analysis results shown below also confirms the presence of the trigger in comparison to the trigger sequences in the reference paper (Green et al. 2014). The biobricked trigger's (BBa_K2550001) function has been validated through experimentation and sequencing.
Figure 1: Image showing the sequencing results of the T7 Toehold LacZ RNA Trigger Sequence. Annotated in a white arrow is the Biobricked T7 promoter (BBa_I719005); this is followed by the Biobricked trigger sequence, which is 182 base pairs long, starting at 50 bp and ending at 231 bp.
The illustration justifies that the trigger sequence is present along with the T7 promoter.
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