Difference between revisions of "Part:BBa K2804005"
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The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.
 
The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.
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<div class="ec--h3"><a name="SF">CBD cipA-sfGFP</a></div>
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                    <p><img src="https://static.igem.org/mediawiki/2018/e/e4/T--Ecuador--CBD-sfGFP.png" width="268" height="261"/></p>
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                      <p><strong>Figure 2. </strong>Tridimensional structure of CBD cipA-sfGFP  obtained from the server I-Tasser.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:15, 18 October 2018


CBD cipA fused to sfGFP under the control of LacI promoter

The superfolder green fluorescent protein (sfGFP) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]