Difference between revisions of "Part:BBa K2610032"
JazzyDeWaard (Talk | contribs) |
|||
| (2 intermediate revisions by one other user not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2610032 short</partinfo> | <partinfo>BBa_K2610032 short</partinfo> | ||
| − | This composite part features the regulatory part promoter SoxS (BBa_K2610030) and the fluorescent protein mCherry ( | + | This composite part features the regulatory part promoter SoxS ([https://parts.igem.org/Part:BBa_K2610030 BBa_K2610030]) and the fluorescent protein mCherry ([https://parts.igem.org/Part:BBa_J06504 BBa_J06504]). It can be used to visualize upregulation of SoxS as a result of superoxide stress. |
| − | + | ||
| − | + | ||
| + | Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence. | We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence. | ||
| + | [[File:T--Leiden--SoxS-mcherry.png|750px]] <br> | ||
| + | <span style="font-size:1em"><b>Figure 1.</b> pSoxS-mCherry transformed cells untreated (A and B) and treated with 100 ug/mL nalidixic acid. </span> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:11, 18 October 2018
pSoxS-mCherry
This composite part features the regulatory part promoter SoxS (BBa_K2610030) and the fluorescent protein mCherry (BBa_J06504). It can be used to visualize upregulation of SoxS as a result of superoxide stress.
Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system.
Usage and Biology
We, iGEM Leiden 2018, have used confocal microscopy in order to visualize the E.coli stress response to nalidixic acid. We incubated our transformed DH5a cells with 100 ug/mL nalidixic acid for 3 hours, after which they were analysed with the confocal (Figure 1). As can be observed, treating the cells with nalidixic acid resulted in increased fluorescence.
Figure 1. pSoxS-mCherry transformed cells untreated (A and B) and treated with 100 ug/mL nalidixic acid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]