Difference between revisions of "Part:BBa K2656000"
 
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<partinfo>BBa_K2656000 short</partinfo>
 
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[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]]
  
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Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005  BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [[File:https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf| Golden Gate assembly protocol]].
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Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005  BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 01:05, 18 October 2018


T7 phage strong promoter

domestication.
Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication.


Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase BBa_I719005 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]