Difference between revisions of "Part:BBa K2656000"
 
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<partinfo>BBa_K2656000 short</partinfo>
 
<partinfo>BBa_K2656000 short</partinfo>
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Part BBa_I719005 domesticated to the Golden Braid 3.0 grammar
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[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]]
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Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins. It is domesticated to the Golden Braid 3.0 grammar. It can be used to assembly transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.
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Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005  BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 01:05, 18 October 2018


T7 phage strong promoter

domestication.
Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication.


Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase BBa_I719005 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]