Difference between revisions of "Part:BBa K2381012"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2381012 short</partinfo>
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<partinfo>BBa_K2381012 short</partinfo>  
  
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It's a gRNA targeting towards OriC
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<p>
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It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/>
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The targeting sequence of this part is acquired from reference paper<sup>1</sup>.
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<img src="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="750px"/>
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<b>BBa_K2381024</b>, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.
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<img src="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png" width="750px"/>
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Further investigation is conducted by qPCR and flow cytometric.
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</br>
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<img src="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png" width="750px"/>
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Its function after light-induced promoter, <b>BBa_K2381023</b>, is proved by OD measurement.
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<h3>References</h3>
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<p>
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1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2381012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2381012 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
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Latest revision as of 01:00, 18 October 2018


3'-OriC gRNA

It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from reference paper1.


BBa_K2381024, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.



Further investigation is conducted by qPCR and flow cytometric.



Its function after light-induced promoter, BBa_K2381023, is proved by OD measurement.


References

1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]