Difference between revisions of "Part:BBa K2738104"

Latest revision as of 00:40, 18 October 2018

Enterocin fused to N-ter designed protein lyase cage monomer

Part: BBa_K2738104 (designed protein lyase cage-enterocin A)

Description We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.

The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 743
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2738104 short</partinfo>
-Enterocin is an AMP
+Part: BBa_K2738104 (designed protein lyase cage-enterocin A)
-Fusion protein
-Protein lyase cage is designed synthetically to form a multimer
+Description
+We aimed to fuse antimicrobial peptides to self-assembling multimeric proteins giving us star-shape antimicrobial peptides called StarCores. The StarCore described here is a result of the fusion of protein-lyase-cage (self-assembling protein) to Enterocin A (antimicrobial peptide) via golden-gate assembly. The fusion protein expression is regulated by T7-promoter.
+The protein-lyase-cage is a multimeric, self-assembling protein formed by synthetically designed monomeric subunits. Enterocin A is an antimicrobial peptide, naturally produced by Enterococcus faecium.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===