Difference between revisions of "Part:BBa K2560086:Design"

 
 
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===Design Notes===
 
===Design Notes===
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More information coming soon!
 
  
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.
  
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===Source===
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</html>
  
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More information coming soon!
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<b> Forward Oligo:</b>
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CTCGGCTTTACACCATCACCACCATCATGGGTA
  
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===References===
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<b> Reverse Oligo:</b>
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CTCATACCCATGATGGTGGTGATGGTGTAAAGC

Latest revision as of 00:33, 18 October 2018


Phytobrick version of 5a His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGGCTTTACACCATCACCACCATCATGGGTA

Reverse Oligo: CTCATACCCATGATGGTGGTGATGGTGTAAAGC