Difference between revisions of "Part:BBa K2587018"
 
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<partinfo>BBa_K2587018 short</partinfo>
 
<partinfo>BBa_K2587018 short</partinfo>
  
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This construct contains the lysis gene <i>E</I>. <i>E</I> is a lysis gene from the bacteriophage PhiX174. This gene is used in our co-culture to induce self lysis of <i>E. coli</i> under the control of the P<i><sub>rpa</sub></i> promoter. This promoter is part of the Quorum sensing system of <i>Rhodopseudomonas palustris</i>. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.
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This construct contains the lysis gene <i>E</I>. <i>E</I> is a lysis gene from the bacteriophage phiX174. This gene is used in our co-culture to induce self lysis of <i>E. coli</i> under the control of the P<i><sub>rpa</sub></i> promoter. This promoter is part of the quorum sensing system of <i>Rhodopseudomonas palustris</i>. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.
  
 
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Latest revision as of 00:33, 18 October 2018


Prpa_RBS_E_T

This construct contains the lysis gene E. E is a lysis gene from the bacteriophage phiX174. This gene is used in our co-culture to induce self lysis of E. coli under the control of the Prpa promoter. This promoter is part of the quorum sensing system of Rhodopseudomonas palustris. Together with the other components of the system, RpaR and RpaI, this promoter induces expression of the lysis gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93