Difference between revisions of "Part:BBa K2680068"
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===Mechanics=== | ===Mechanics=== | ||
| − | In this circuit, temperature-sensitive CI is expressed constitutively by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is | + | In this circuit, temperature-sensitive CI is expressed constitutively by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is raised to approximately 37, the ts-CI protein denatures, losing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of the rapidly-maturing mScarlet-I. |
Revision as of 00:31, 18 October 2018
J23107 ts-CI + R0051 mScarlet-I
37C Temperature Sensitive mScarlet-I
Temperature-Sensitive mScarlet-I. Components: J23107 ts-CI + R0051 mScarlet-I
Mechanics
In this circuit, temperature-sensitive CI is expressed constitutively by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is raised to approximately 37, the ts-CI protein denatures, losing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of the rapidly-maturing mScarlet-I.
Role in an IFFL System
While this circuit is not an IFFL, it acts as a control to determine the normal rate of expression of mScarlet-I given interaction between the ts-CI and the CI promoter as temperature changes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 74
Illegal NotI site found at 1518 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]