Difference between revisions of "Part:BBa K2656019"

Revision as of 00:26, 18 October 2018

LuxI Coding Sequence

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Gate] assembly methods. It was codon optimized for E. coli.

LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.

This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.
-This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [https://static.igem.org/mediawiki/2018/b/bc/T--Valencia_UPV--protocols.pdf Golden Gate assembly protocol].
+This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
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