Difference between revisions of "Part:BBa K2587012"
Line 3: | Line 3: | ||
<partinfo>BBa_K2587012 short</partinfo> | <partinfo>BBa_K2587012 short</partinfo> | ||
− | This part contains LuxR (BBa_K2587003:https://parts.igem.org/Part:BBa_K2587003) as a complete | + | This part contains LuxR (BBa_K2587003:https://parts.igem.org/Part:BBa_K2587003) as a complete transcriptional unit with a constitutive promoter, RBS and Terminator. |
− | LuxR is a component of the quorum sensing system and is used as a regulator, forming a complex with the quorum sensing molecule and binding to the promoter | + | LuxR is a component of the quorum sensing system and is used as a regulator, forming a complex with the quorum sensing molecule and binding to the promoter P<sub><i>lux</i></sub> which induces synthesis of the target gene. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 00:06, 18 October 2018
P_RBS_luxR_T
This part contains LuxR (BBa_K2587003:https://parts.igem.org/Part:BBa_K2587003) as a complete transcriptional unit with a constitutive promoter, RBS and Terminator. LuxR is a component of the quorum sensing system and is used as a regulator, forming a complex with the quorum sensing molecule and binding to the promoter Plux which induces synthesis of the target gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 260
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 70
Illegal BsaI.rc site found at 701