Difference between revisions of "Part:BBa K2560303"
 
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Latest revision as of 23:48, 17 October 2018


FapR, MaCoA Sensor part. This part is a PhytoBrick variant


This part contains the coding sequence for FapR. It is expressed via a constitutive promoter. FapR is a repressor of the fapO site. When FapR binds Malonyl-CoA it leaves the fapO site and transcription can occur.


Usage and Biology

  • This part acts as a repressor controlling the expression of any part controlled by fapO. (K2560302)
  • Malonyl-CoA is a normal metabolite and completely harmless. For induction, a concentration between 2 and 25 mM can be used.
  • The system was taken from B. subtilis 168
  • We designed this biobrick in order to get a system with a usable readout for the intracellular Malonyl-CoA concentration.
  • Can with be combined with a several reporter genes. We had the best results using the LUX operon.


' Figure 1
This is a schematic, illustrating the working mechanism of the Malonyl-CoA sensor
File:T--Marburg--SensorConstructMalonylCoA-01.png
Figure 1
This is a schematic, illustrating the genetic context and assembly of the Malonyl-CoA sensor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]