Difference between revisions of "Part:BBa K2765021:Design"
Line 8: Line 8:
  
 
we constructed an expression plasmid based on pESC-Leu, ligase gene yno1 into it, We use <i>E.coli</i> as our host cell to construct the  
 
we constructed an expression plasmid based on pESC-Leu, ligase gene yno1 into it, We use <i>E.coli</i> as our host cell to construct the  
−
rcombinant plasmid, transformed it into <i>Saccharomyces cerevisiae</i>.
+
rcombinant plasmid, then transformed it into <i>Saccharomyces cerevisiae</i>.
  
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]]
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]]
  
 
Here shows the result of Colony PCR in <i>E.coli</i>.
 
Here shows the result of Colony PCR in <i>E.coli</i>.

Revision as of 23:40, 17 October 2018

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

T--BIT-China--iGEM2018-Partsregulator-4.png

Obtained gene yno1 though clone from yeast genome.

T--BIT-China--iGEM2018-Partsregulator-5.png

we constructed an expression plasmid based on pESC-Leu, ligase gene yno1 into it, We use E.coli as our host cell to construct the rcombinant plasmid, then transformed it into Saccharomyces cerevisiae.

T--BIT-China--iGEM2018-Partsregulator-6.png

Here shows the result of Colony PCR in E.coli.