Difference between revisions of "Part:BBa K2832005"
Alexkata15 (Talk | contribs) |
|||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2832005 short</partinfo> | <partinfo>BBa_K2832005 short</partinfo> | ||
| − | + | General stress response sigma factor. Responds to stress on membrane and periplasmic proteins. Binding of unassembled outer membrane proteins to the protease DegS stimulate its cleavage of the regulatory protein RseA, which tethers sigma E to the inner membrane. bamE is a member of the sigma E regulon. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates the expression of RFP. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 23:39, 17 October 2018
RpoE Inducible mRFP1 Generator (RpoE --> bamEp --> mRFP1)
General stress response sigma factor. Responds to stress on membrane and periplasmic proteins. Binding of unassembled outer membrane proteins to the protease DegS stimulate its cleavage of the regulatory protein RseA, which tethers sigma E to the inner membrane. bamE is a member of the sigma E regulon. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it stimulates the expression of RFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 13
Illegal AgeI site found at 769
Illegal AgeI site found at 881 - 1000COMPATIBLE WITH RFC[1000]