Difference between revisions of "Part:BBa K2787029"
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Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033). | Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033). | ||
− | + | ==<b>Usage and Biology</b>== | |
− | + | [[File:ShanghaiTech2018-pT181-1.png|700px|center]] | |
+ | [[File:ShanghaiTech2018-pT181-2.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-3.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-4.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-5.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-6.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-7.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-8.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-9.png|700px|center]] | ||
+ | [[File:ShanghaiTech2018-pT181-10.png|700px|center]] | ||
+ | |||
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Revision as of 23:37, 17 October 2018
RNA attenuator pT181 with GFP under pT181 target sequence by Kyoto 2013 iGEM Team
Measurement of repression efficiency of pT181 sense target submitted by Kyoto 2013 iGEM team(BBa_K1126003) under the expression kit(BBa_K2787027) we designed to test the functionality of our improved version of pT181 sense target(BBa_K2787030). This part is identical to BBa_K2787027 except for the pT181 sense target is changed to BBa_K1126003 and a RBS(BBa_K1893033).
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 268
Illegal NheI site found at 291 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1247