Difference between revisions of "Part:BBa K2765050"

 
 
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<partinfo>BBa_K2765050 short</partinfo>
 
<partinfo>BBa_K2765050 short</partinfo>
  
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This part is the expression cassette of the roGFP2-orp1 .We completed roGFP2-orp1 expression cassette started by the promoter TEF1.
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This part consists of the promoter TEF2p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter TEF2p is an constitutive promoter from <i>Saccharomyces cerevisiae</i> CEN.PK-1c（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).
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It can express green fluorescent protein roGFP2-orp1&#65292;and this protein can reflect the accumulation of ROS in cells.
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[[Image: T--BIT-China--iGEM2018-PartsOutput-TEF2geneline.1.png |center|400px|]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:33, 17 October 2018


Complete roGFP2-Orp1 expression cassette started by the promoter TEF2

This part consists of the promoter TEF2p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter TEF2p is an constitutive promoter from Saccharomyces cerevisiae CEN.PK-1c（see the details in part：BBa_K124001）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

T--BIT-China--iGEM2018-PartsOutput-TEF2geneline.1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1564