Difference between revisions of "Part:BBa K914000"

Latest revision as of 23:22, 17 October 2018

pLac-supD-T

This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here). This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.

This part has been improved by part BBa_K2725016 - supD expressed under J23108 anderson promotor (strength 0.51). This expression level of supD allows the expression of a gene with 5 serine codons substituted for amber STOP codons.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 96
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 99

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K914000 short</partinfo>
 This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
-This part is used to allow the expression of gene that have one or many amber codon instead of a Serine. Adding IPTG could be usefull when there are to many amber codon in the cell.
+This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.
+This part has been improved by part BBa_K2725016 - supD expressed under J23108 anderson promotor (strength 0.51). This expression level of supD allows the expression of a gene with 5 serine codons substituted for amber STOP codons.
 <!-- Add more about the biology of this part here