Difference between revisions of "Part:BBa K2675066"

Latest revision as of 23:19, 17 October 2018

sfGFP expression cassette under the control of J23110 promoter

This part is an sfGFP (BBa_K2675005) expression cassette under the control of the constitutive promoter BBa_J23110.

Usage and Biology

In this composite part sfGFP (BBa_K2675005) was equipped by a custom made RBS (BBa_K2675017) and placed under the control of the constitutive promoter (BBa_J23110).

The pictures of E. coli cells harbouring this part show a very strong sfGFP expression. In contrast, no sfGFP is observed in the absence of the promoter and the RBS (BBa_K2675080).

Figure 1. Pictures of E. coli cells harbouring an empty pSB1C3 backbone, BBa_K2675080 or this part BBa_K2675066.

We used this part as a positive control of sfGFP expression and as a negative control of promoter regulation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 90
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 In this composite part sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) was equipped by a custom made RBS ([[Part:BBa_K2675017|BBa_K2675017]]) and placed under the control of the constitutive promoter ([[Part:BBa_J23110|BBa_J23110]]).
-The pictures of ''E. coli'' cells harbouring this part show a very strong sfGFP-LVAtag expression. In contrast, no sfGFP-LVAtag is observed in the absence of the promoter and the RBS ([[Part:BBa_K2675080|BBa_K2675080]]).
+The pictures of ''E. coli'' cells harbouring this part show a very strong sfGFP expression. In contrast, no sfGFP is observed in the absence of the promoter and the RBS ([[Part:BBa_K2675080|BBa_K2675080]]).
 [[File: T--Evry Paris-Saclay--Drops-on-plate BBa K2675080.png|600px]]