Difference between revisions of "Part:BBa K2675056"
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We used this part as a positive control of sfGFP-LVAtag expression and as a negative control of promoter regulation.
 
We used this part as a positive control of sfGFP-LVAtag expression and as a negative control of promoter regulation.
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Revision as of 23:17, 17 October 2018


sfGFP-LVAtag expression casette under the control of J23110 promoter

This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the constitutive promoter BBa_J23110.

Usage and Biology

In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by a custom made RBS (BBa_K2675017) and placed under the control of the constitutive promoter (BBa_J23110).

The pictures of E. coli cells harbouring this part show a very strong sfGFP-LVAtag expression. In contrast, no sfGFP-LVAtag is observed in the absence of the promoter and the RBS (BBa_K2675081).

T--Evry Paris-Saclay--Drops-on-plate BBa K2675081.png

Figure 1. Pictures of E. coli cells harbouring an empty pSB1C3 backbone, BBa_K2675081 or this part BBa_K2675056.

We used this part as a positive control of sfGFP-LVAtag expression and as a negative control of promoter regulation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]