Difference between revisions of "Part:BBa K2675056"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | In this composite part sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) was equipped by a custom made RBS ([[Part:BBa_K2675017|BBa_K2675017]]) and placed under the control of the constitutive promoter ([[Part:BBa_J23110|BBa_J23110]]). | |
The pictures of ''E. coli'' cells harbouring this part show a very strong sfGFP-LVAtag expression by this this part [[Part:BBa_K2675056|BBa_K2675056]] and no sfGFP-LVAtag in the absence of the promoter and the RBS ([[Part:BBa_K2675081|BBa_K2675081]]). | The pictures of ''E. coli'' cells harbouring this part show a very strong sfGFP-LVAtag expression by this this part [[Part:BBa_K2675056|BBa_K2675056]] and no sfGFP-LVAtag in the absence of the promoter and the RBS ([[Part:BBa_K2675081|BBa_K2675081]]). | ||
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Figure 1. Pictures of ''E. coli'' cells harbouring an empty pSB1C3 backbone, [[Part:BBa_K2675081|BBa_K2675081]] or this part [[Part:BBa_K2675056|BBa_K2675056]]. | Figure 1. Pictures of ''E. coli'' cells harbouring an empty pSB1C3 backbone, [[Part:BBa_K2675081|BBa_K2675081]] or this part [[Part:BBa_K2675056|BBa_K2675056]]. | ||
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| + | We used this part as a positive control of sfGFP-LVAtag expression and as a negative control of promoter regulation. | ||
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Revision as of 23:14, 17 October 2018
sfGFP-LVAtag expression casette under the control of J23110 promoter
This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the constitutive promoter BBa_J23110.
Usage and Biology
In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by a custom made RBS (BBa_K2675017) and placed under the control of the constitutive promoter (BBa_J23110).
The pictures of E. coli cells harbouring this part show a very strong sfGFP-LVAtag expression by this this part BBa_K2675056 and no sfGFP-LVAtag in the absence of the promoter and the RBS (BBa_K2675081).
Figure 1. Pictures of E. coli cells harbouring an empty pSB1C3 backbone, BBa_K2675081 or this part BBa_K2675056.
We used this part as a positive control of sfGFP-LVAtag expression and as a negative control of promoter regulation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 90
- 1000COMPATIBLE WITH RFC[1000]