Difference between revisions of "Part:BBa K2686002:Experience"
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| − | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.96kDa for the monomer and 1.98MDa for the 60-mer). DLS measurements also show that our protein cage self-assembles into the 60-mer. | + | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.96kDa for the monomer and 1.98MDa for the 60-mer). DLS measurements also seem to show that our protein cage self-assembles into the 60-mer. |
===Applications of BBa_K2686002=== | ===Applications of BBa_K2686002=== | ||
Latest revision as of 23:12, 17 October 2018
The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.96kDa for the monomer and 1.98MDa for the 60-mer). DLS measurements also seem to show that our protein cage self-assembles into the 60-mer.
Applications of BBa_K2686002
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UNIQ1cb43d453adfc1ee-partinfo-00000001-QINU UNIQ1cb43d453adfc1ee-partinfo-00000002-QINU