Difference between revisions of "Part:BBa K2765021:Design"

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[[Image: T--BIT-China--iGEM2018-Partsregulator-4.png |center|300px|]]
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-4.png |center|300px|]]
  
   
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  Obtained gene yno1 though clone from yeast genome.
  
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center|600px|]]
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center|600px|]]
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[[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]]
 
[[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]]

Revision as of 23:12, 17 October 2018

To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.

T--BIT-China--iGEM2018-Partsregulator-4.png
Obtained gene yno1 though clone from yeast genome.
T--BIT-China--iGEM2018-Partsregulator-5.png


T--BIT-China--iGEM2018-Partsregulator-6.png