Difference between revisions of "Part:BBa K2686002:Design"
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===Design Notes===
 
===Design Notes===
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The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was codon optimized for bacterial expression in ''E. coli'' and introduced in between amino acids 43/44 in [[parts.igem.org/Part:BBa_K2686001|BBa_K2686001]] which is shifted by one amino acid downstream compared to the location in [[parts.igem.org/Part:BBa_K192000|BBa_K192000]] where it would be in between amino acids 42/43 (Moon et al., 2014).
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The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was introduced in between amino acids 43/44 in <bbpart>BBa_K2686001</bbpart> which is shifted by one amino acid downstream compared to the location in <bbpart>BBa_K192000<bbpart> where it would be in between amino acids 42/43 (Moon et al., 2014).
  
 
===Source===
 
===Source===

Latest revision as of 23:11, 17 October 2018


Encapsulin protein with HexaHistidine insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


Design Notes

The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was introduced in between amino acids 43/44 in BBa_K2686001 which is shifted by one amino acid downstream compared to the location in BBa_K192000