Difference between revisions of "Part:BBa K2765021:Design"
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Revision as of 23:07, 17 October 2018
To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.