Difference between revisions of "Part:BBa K2587000"

Latest revision as of 23:01, 17 October 2018

luxI_codon optimized S.cerevisiae

LuxI is an acyl homoserine lactone synthase, most known from the bacterium Aliivibrio fischeri. LuxI is a basic component of the quorum sensing system of A. fischeri. Bacteria use it as a communication module to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, Saccharomyces cerevisiae. Besides the codon optimization, the construct also contains type II S restriction sites (BsaI), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.

Usage and Biology

This part can be used as a component for designing synthetic circuits in S.cerevisiae. For instance, in our project, we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 6
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 689
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 30
Illegal BsaI.rc site found at 673

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 <partinfo>BBa_K2587000 short</partinfo>
-LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Alivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <nobr><i>A. fischeri</I></nobr>. In bacteria use it as a communication module among organisms to regulate expression of genes. In this part the synthase is codon optimized for the common yeast, <nobr><i>Saccharomyces cerevisiae</I></nobr>. Besides the codon optimization the construct contains also type II S cutting sites (BsaI), useful for example for Golden Gate cloning method. In yeast quorum sensing system is not frequently encountered and therefore a codon optimized form for utilization in an eukaryotic organism represents a beginning to design control systems in this type of organisms. For example, this LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter or a lysis gene to control cell population.
+LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Aliivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <i>A. fischeri</I>. Bacteria use it as a communication module  to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, <i>Saccharomyces cerevisiae</I>. Besides the codon optimization, the construct also contains type II S restriction sites (<i>Bsa</i>I), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.
 <b>Usage and Biology</b>
-This part can be used as a component for designing synthetic circuits in <i>S.cerevisiae</i>. For instance in our project we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.
+This part can be used as a component for designing synthetic circuits in <i>S.cerevisiae</i>. For instance, in our project, we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.
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