Difference between revisions of "Part:BBa K2533039"

 
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<h1>'''Usage and biology'''</h1>
 
<h1>'''Usage and biology'''</h1>
gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO2. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced.
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gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO<sub>2</sub>. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced.
  
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is the whole circuit for NADH production part.
 
This is the whole circuit for NADH production part.
[[File:T--HUST-China--2018-tonglu-gapA-mdh-pflB-fdh.png ‎|400px|thumb|center|Figure1:The whole circuit]]
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[[File:T--HUST-China--2018-tonglu-gapA-mdh-pflB-fdh.png ‎|400px|thumb|center|Figure1. The whole circuit]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 22:58, 17 October 2018


RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh

It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.

Usage and biology

gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO2. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced.

Characterization

This is the whole circuit for NADH production part.

Figure1. The whole circuit