Difference between revisions of "Part:BBa K2533039"
| (2 intermediate revisions by 2 users not shown) | |||
| Line 5: | Line 5: | ||
It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b. | It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b. | ||
| − | < | + | <h1>'''Usage and biology'''</h1> |
| − | + | gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO<sub>2</sub>. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| + | <h1>'''Characterization'''</h1> | ||
| + | This is the whole circuit for NADH production part. | ||
| + | [[File:T--HUST-China--2018-tonglu-gapA-mdh-pflB-fdh.png |400px|thumb|center|Figure1. The whole circuit]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K2533030 parameters</partinfo> |
<!-- --> | <!-- --> | ||
Latest revision as of 22:58, 17 October 2018
RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh
It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.
Usage and biology
gapA could transform 3- phosphoglyceraldehyde into 1,3- diphosphoglycerate, mdh could transform malate into pyruvate, pflB helps to transform pyruvate into Acetyl-CoA and fdh helps to transform formate into CO2. With the overexpression of gapA-mdh, Shewanella could produce NADH more efficiently, which brings more electricity to be produced.
Characterization
This is the whole circuit for NADH production part.
