Difference between revisions of "Part:BBa K2533031"

 
 
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<partinfo>BBa_K2533031 short</partinfo>
 
<partinfo>BBa_K2533031 short</partinfo>
  
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produce L-lactate dehydrogenase complex protein.
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It encodes L-lactate dehydrogenase.
  
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<h1>'''Usage and biology'''</h1>
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===Usage and Biology===
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lldEFG refers to three genes: lldE, lldF and lldG. They could encode a L-lactate dehydrogenase complex which could catalyze L-lactate’s transformation into pyruvate.
  
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<h1>'''Characterization'''</h1>
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<span class='h3bb'>Sequence and Features</span>
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This is one section for lactate utilization part.
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<partinfo>BBa_K2533031 SequenceAndFeatures</partinfo>
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[[File:T--HUST-China--2018-tonglu-lldEFG.png ‎|400px|thumb|center|Figure1. RBS-lldEFG]]
  
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<h2>DNA Gel Electrophoretic</h2>
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To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
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[[File:T--HUST-China--2018-Notebook-gel9.jpeg|400px|thumb|center|Figure2. Verification of successful transformation of pYYDT-lldEFG]]
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Our target gene is 3037bp, and as the marker is DS5000, we could be sure that the bright band in this picture is our target gene.
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<h2>Electrogenesis</h2>
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By comparing the ability of producing electricity, we might find out whether lldEFG could effectively help Shewanella to produce more electricity.
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[[File:T--HUST-China--2018-elec-dld.png ‎|400px|thumb|center|Figure3. The comparison of electricity production between Shewanella contained pYYDT and pYYDT-lldEFG.]]
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It could be demonstrated that targeted gene could be expressed in the engineered cells. More lactate has been utilized by engineered bacteria, which helps to produce more electricty.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2533031 parameters</partinfo>
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<partinfo>BBa_K2533030 parameters</partinfo>
 
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Latest revision as of 22:52, 17 October 2018


RBS-lldEFG

It encodes L-lactate dehydrogenase.

Usage and biology

lldEFG refers to three genes: lldE, lldF and lldG. They could encode a L-lactate dehydrogenase complex which could catalyze L-lactate’s transformation into pyruvate.

Characterization

This is one section for lactate utilization part.

Figure1. RBS-lldEFG

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2. Verification of successful transformation of pYYDT-lldEFG

Our target gene is 3037bp, and as the marker is DS5000, we could be sure that the bright band in this picture is our target gene.

Electrogenesis

By comparing the ability of producing electricity, we might find out whether lldEFG could effectively help Shewanella to produce more electricity.

Figure3. The comparison of electricity production between Shewanella contained pYYDT and pYYDT-lldEFG.

It could be demonstrated that targeted gene could be expressed in the engineered cells. More lactate has been utilized by engineered bacteria, which helps to produce more electricty.