Difference between revisions of "Part:BBa K2787013"

 
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The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.
 
The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.
  
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===Usage and Biology===
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===Usage and Biology===
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[[File:ShanghaiTech2018-b8 rbs.png|700px|center]]
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[[File:ShanghaiTech-TJU_16S.png|700px|center]]
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[[File:A2-B8-C9-Orthogonality.png|700px|center]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:48, 17 October 2018


GFP under B8 orthogonal RBS with orthogonal 16S rRNA BBa_K2787041

The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the TJU and B8 ribosome system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 798
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707