Difference between revisions of "Part:BBa K2787010"
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The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the A2 and B8 ribosome system. | The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the A2 and B8 ribosome system. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | [[File:ShanghaiTech2018-b8 rbs.png|700px|center]] | ||
+ | [[File:A2-B8-C9-Orthogonality.png|700px|center]] | ||
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Revision as of 22:44, 17 October 2018
GFP under B8 orthogonal RBS with orthogonal 16S rRNA A2
The 16S rRNA A2 under constitutive promoter BBa_J23100(BBa_K2787006) and pCons-oRBSB8-GFP(BBa_K2787001) are linked into one plasmid to quantify the orthogonality of the A2 and B8 ribosome system.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 798
Illegal NheI site found at 821 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1660
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707