Difference between revisions of "Part:BBa K2859000:Experience"
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| + | Afther synthesizing the part flanked by biobrick prefix and suffix, we used digested the part and pSB1C3 vector with EcoR1 and Pst1 and ligated the part into the pSB1C3 vector. The resulting plasmid was transformed into E.Coli DH5-α. They were grown overnight at 37°C. The samples were prepared for SDS page gel. The expression of Penetratin-Insulin-Flag tag complex was evaluated with SDS page gel. | ||
https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg | https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg | ||
Revision as of 22:42, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2859000
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UNIQ1a7ab77f2cb3149b-partinfo-00000000-QINU UNIQ1a7ab77f2cb3149b-partinfo-00000001-QINU
Afther synthesizing the part flanked by biobrick prefix and suffix, we used digested the part and pSB1C3 vector with EcoR1 and Pst1 and ligated the part into the pSB1C3 vector. The resulting plasmid was transformed into E.Coli DH5-α. They were grown overnight at 37°C. The samples were prepared for SDS page gel. The expression of Penetratin-Insulin-Flag tag complex was evaluated with SDS page gel.
