Difference between revisions of "Part:BBa K2570021:Design"

(→‎Design Notes)
(→‎References)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
This is an improved version of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2120002 BBa_K2120002].
 +
 
dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.
 
dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.
  
Line 17: Line 19:
  
 
===References===
 
===References===
−
1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.
+
 
 +
[1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.
 +
 
 +
[2] Sledjeski, D. D., A. Gupta, and S. Gottesman. 1996. The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli. EMBO J. 15:3993–4000
 +
 
 +
[3] Majdalani, N., C. Cunning, D. Sledjeski, T. Elliott, and S. Gottesman. 1998. DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription. Proc. Natl. Acad. Sci. USA 95:12462–12467.
 +
 
 +
[4] Repoila, F., and S. Gottesman. 2001. Signal transduction cascade for regulation of RpoS: temperature regulation of dsrA. J. Bacteriol. 183:4012–4023.

Latest revision as of 22:38, 17 October 2018


dsrA+B0034+GFP+B0032+mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


Design Notes

This is an improved version of BBa_K2120002.

dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.

We obtained the wild-type mazf toxin protein sequence from E. coli and conducted experiments as a positive control.

Source

It comes from E.coli.


References

[1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.

[2] Sledjeski, D. D., A. Gupta, and S. Gottesman. 1996. The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli. EMBO J. 15:3993–4000

[3] Majdalani, N., C. Cunning, D. Sledjeski, T. Elliott, and S. Gottesman. 1998. DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription. Proc. Natl. Acad. Sci. USA 95:12462–12467.

[4] Repoila, F., and S. Gottesman. 2001. Signal transduction cascade for regulation of RpoS: temperature regulation of dsrA. J. Bacteriol. 183:4012–4023.