Difference between revisions of "Part:BBa K2570021:Design"

 
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===Design Notes===
 
===Design Notes===
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This is an improved version of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2120002 BBa_K2120002].
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dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.
 
dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.
  
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+
We obtained the wild-type mazf toxin protein sequence from E. coli and conducted experiments as a positive control.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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[1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.
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[2] Sledjeski, D. D., A. Gupta, and S. Gottesman. 1996. The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli. EMBO J. 15:3993–4000
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[3] Majdalani, N., C. Cunning, D. Sledjeski, T. Elliott, and S. Gottesman. 1998. DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription. Proc. Natl. Acad. Sci. USA 95:12462–12467.
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[4] Repoila, F., and S. Gottesman. 2001. Signal transduction cascade for regulation of RpoS: temperature regulation of dsrA. J. Bacteriol. 183:4012–4023.

Latest revision as of 22:38, 17 October 2018


dsrA+B0034+GFP+B0032+mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


Design Notes

This is an improved version of BBa_K2120002.

dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.

We obtained the wild-type mazf toxin protein sequence from E. coli and conducted experiments as a positive control.

Source

It comes from E.coli.


References

[1] Repoila, F. and S. Gottesman (2003). "Temperature Sensing by the dsrA Promoter." Journal of Bacteriology 185(22): 6609-6614.

[2] Sledjeski, D. D., A. Gupta, and S. Gottesman. 1996. The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli. EMBO J. 15:3993–4000

[3] Majdalani, N., C. Cunning, D. Sledjeski, T. Elliott, and S. Gottesman. 1998. DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription. Proc. Natl. Acad. Sci. USA 95:12462–12467.

[4] Repoila, F., and S. Gottesman. 2001. Signal transduction cascade for regulation of RpoS: temperature regulation of dsrA. J. Bacteriol. 183:4012–4023.