Difference between revisions of "Part:BBa K2765053"

Revision as of 22:33, 17 October 2018

Complete roGFP2-Orp1 expression cassette started by the promoter FBA1t.

This part consists of the promoter FBA1p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter FBA1p is an constitutive promoter from Saccharomyces cerevisiae Cen.PK2-1c（see the details in part：BBa_K2765040）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

File:T--BIT-China--iGEM2018-PartsOutput-FBA1p-geneline.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1480
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1792

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2765053 short</partinfo>
-This part consists of the promoter FBA1p , redox sensor roGFP2-Orp1 and the terminator cyc1. The promoter FBA1p is an constitutive promoter from <i>Saccharomyces cerevisiae Cen.PK2-1c</i>（see the details in part：BBa_K2765040）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).
+This part consists of the promoter FBA1p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter FBA1p is an constitutive promoter from <i>Saccharomyces cerevisiae Cen.PK2-1c</i>（see the details in part：BBa_K2765040）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).
 [[Image: T--BIT-China--iGEM2018-PartsOutput-FBA1p-geneline.png |center|400px|]]